Effect of lron Deficiency on the Respiration of Sycamore
نویسنده
چکیده
The effects of iron deficiency on cell culture growth, cell respiration, mitochondrial oxidative properties, and the eledron transport chain were studied with suspension-cultured sycamore (Acer pseudoplatanus 1.) cells. lron deprivation considerably decreased the initial growth rates and limited the maximum density of the cells. Under these conditions, the cells remained swollen throughout their growth. The absence of iron led to a steady decline in the uncoupled rate of Oz consumption. When the uncoupled rate of 0 2 uptake closely approximated the respiratory rate, the cells began to collapse. At this stage, the leve1 of all the cytochromes and eledron paramagnetic resonance-detedable Fe-S clusters of the mitochondrial inner membrane were dramatically decreased. Nevertheless, it appeared from substrate oxidation measurements that this overall depletion in iron-containing components solely disturbed the fundioning of complex II, whereas neither complexes I, 111, or IV, nor the machinery involved in ATP synthesis, was apparently impaired in iron-deficient mitochondria. However, our results suggest that the impairment of complex I I resulted in a strong reduction of the overall capacity of the mitochondrial electron transport chain, which was responsible for determining the rate of endogenous respiration in sycamore cells. Finally, this situation led to a depletion of various energy metabolites that could contribute to the premature cell death.
منابع مشابه
Mitochondria of Isolated Plant Cells (Acer pseudoplatanus L.): II. Copper Deficiency Effects on Cytochrome C Oxidase and Oxygen Uptake.
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تاریخ انتشار 2002